trap primer mixture (TaKaRa)
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TaKaRa
trap primer mixture
Trap Primer Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1452 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trap+primer+mix/Universal+Primer+Mix/10__1021_slash_acs__jmedchem__0c01656-312-51-72
Average 99 stars, based on 1452 article reviews
Trap Primer Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1452 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trap+primer+mix/Universal+Primer+Mix/10__1021_slash_acs__jmedchem__0c01656-312-51-72
Average 99 stars, based on 1452 article reviews
trap primer mixture - by Bioz Stars,
2026-10
99/100 stars
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Incubation:Article Title: Design, synthesis of 4,5-diazafluorene derivatives and their anticancer activity via targeting telomeric DNA G-quadruplex Article Snippet: In our work, 19 novel 4,5-diazafluorene derivatives (11a-d, 12a-d, 13a-d, 14a-c, 15c, 16ac) bearing a 1,3-disubstituted pyrazol/thioxothiazolidinone or thioxothiazolidinone-oxadiazole moieties were designed, synthesized, preliminarily explored for their antitumor activities and in vitro mechanism.. All compounds showed different values of antiproliferative activity against A549, AGS, HepG2 and MCF-7 cell lines through CCK-8.. Especially, the compound 14c exhibited the strongest activity and best selectivity against A549 cells with an IC50 1.13 μM and an SI value of 7.01 relative to MRC-5 cells, which was better than cisplatin (SI = 1.80) as a positive control. Polymerase Chain Reaction:Article Title: TERT polymorphisms modify the risk of acute lymphoblastic leukemia in Chinese children. Article Snippet: Telomerase activity was measured using TRAPeze telomerase detection kit (S7700; Millipore, Bedford, MA) according to manufacturer instructions. .. Briefly, lysates containing 300 ng protein were added into the PCR master mix containing 10× telomeric repeat amplification protocol (TRAP) reaction buffer, 50× deoxynucleoside triphosphate mix, TS primer, Article Title: A mRNA-Responsive G-Quadruplex-Based Drug Release System. Article Snippet: .. In the second step, telomerase reaction products were amplified in a 10-μL PCR reaction mixture containing 50-fold diluted telomerase mixture, 0.2 μL TS primer, 0.2 μL Article Title: A mRNA-Responsive G-Quadruplex-Based Drug Release System Article Snippet: .. In the second step, telomerase reaction products were amplified in a 10-μL PCR reaction mixture containing 50-fold diluted telomerase mixture, 0.2 μL TS primer, 0.2 μL Article Title: In vitro assays predictive of telomerase inhibitory effect of G-quadruplex ligands in cell nuclei. Article Snippet: G-quadruplex-binding and telomerase-inhibiting capacities of G-quadruplex ligands were examined under a cell nuclei-mimicking condition including excess double-stranded DNA (λ DNA) and molecular crowding cosolute (PEG 200).. Under the cell nuclei-mimicking condition, a cationic porphyrin (TMPyP4) did not bind to the G-quadruplex despite the high affinity (Ka = 3.6 × 10 6 M−1) under a diluted condition without λ DNA and PEG 200.. Correspondingly, TMPyP4 inhibited telomerase activity under the diluted condition (IC50 = 1.6 μM) but not under the cell nuclei-mimicking condition. Amplification:Article Title: TERT polymorphisms modify the risk of acute lymphoblastic leukemia in Chinese children. Article Snippet: Telomerase activity was measured using TRAPeze telomerase detection kit (S7700; Millipore, Bedford, MA) according to manufacturer instructions. .. Briefly, lysates containing 300 ng protein were added into the PCR master mix containing 10× telomeric repeat amplification protocol (TRAP) reaction buffer, 50× deoxynucleoside triphosphate mix, TS primer, Article Title: A mRNA-Responsive G-Quadruplex-Based Drug Release System. Article Snippet: .. In the second step, telomerase reaction products were amplified in a 10-μL PCR reaction mixture containing 50-fold diluted telomerase mixture, 0.2 μL TS primer, 0.2 μL Article Title: A mRNA-Responsive G-Quadruplex-Based Drug Release System Article Snippet: .. In the second step, telomerase reaction products were amplified in a 10-μL PCR reaction mixture containing 50-fold diluted telomerase mixture, 0.2 μL TS primer, 0.2 μL Article Title: In vitro assays predictive of telomerase inhibitory effect of G-quadruplex ligands in cell nuclei. Article Snippet: G-quadruplex-binding and telomerase-inhibiting capacities of G-quadruplex ligands were examined under a cell nuclei-mimicking condition including excess double-stranded DNA (λ DNA) and molecular crowding cosolute (PEG 200).. Under the cell nuclei-mimicking condition, a cationic porphyrin (TMPyP4) did not bind to the G-quadruplex despite the high affinity (Ka = 3.6 × 10 6 M−1) under a diluted condition without λ DNA and PEG 200.. Correspondingly, TMPyP4 inhibited telomerase activity under the diluted condition (IC50 = 1.6 μM) but not under the cell nuclei-mimicking condition. TRAP Assay:Article Title: In vitro assays predictive of telomerase inhibitory effect of G-quadruplex ligands in cell nuclei. Article Snippet: G-quadruplex-binding and telomerase-inhibiting capacities of G-quadruplex ligands were examined under a cell nuclei-mimicking condition including excess double-stranded DNA (λ DNA) and molecular crowding cosolute (PEG 200).. Under the cell nuclei-mimicking condition, a cationic porphyrin (TMPyP4) did not bind to the G-quadruplex despite the high affinity (Ka = 3.6 × 10 6 M−1) under a diluted condition without λ DNA and PEG 200.. Correspondingly, TMPyP4 inhibited telomerase activity under the diluted condition (IC50 = 1.6 μM) but not under the cell nuclei-mimicking condition. Electrophoresis:Article Title: In vitro assays predictive of telomerase inhibitory effect of G-quadruplex ligands in cell nuclei. Article Snippet: G-quadruplex-binding and telomerase-inhibiting capacities of G-quadruplex ligands were examined under a cell nuclei-mimicking condition including excess double-stranded DNA (λ DNA) and molecular crowding cosolute (PEG 200).. Under the cell nuclei-mimicking condition, a cationic porphyrin (TMPyP4) did not bind to the G-quadruplex despite the high affinity (Ka = 3.6 × 10 6 M−1) under a diluted condition without λ DNA and PEG 200.. Correspondingly, TMPyP4 inhibited telomerase activity under the diluted condition (IC50 = 1.6 μM) but not under the cell nuclei-mimicking condition. |